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Acinetobacter baylyi gene cluster ACIAD1969-ACIAD1952 contributes to potassium tellurite resistance and twitching motility

by Arguello, Joshua D.

Abstract

Acinetobacter baylyi strain ADP1 is a gram-negative bacterium normally studied because of its high competence for genetic transformation and its ability to catabolize plant-derived aromatic compounds. A previous study has identified that the gene cluster ACIAD1969-ACIAD1952 contains genes that may be responsible for potassium tellurite resistance, as well as other proteins that are “hypothetical.” Our goal was to use bioinformatics to investigate this gene cluster and to determine whether it played a role in potassium tellurite resistance as well as twitching motility. Our results indicate that the gene cluster is actually composed of four different operons that play a role in tellurite resistance. We also found that the gene cluster was most likely inherited from horizontal gene transfer, as it is not found in any other Acinetobacter strains. Furthermore, all genes except ACIAD1956, ACIAD1962 and ACIAD1964 are responsible for potassium tellurite resistance in ADP1 and all mutants exhibit twitching motility defects. Our results indicate that the genes in the gene cluster ACIAD1969-ACIAD1952 encode proteins and should no longer be considered “hypothetical.”

Note

The author has given permission for this work to be deposited in the Digital Archive of Colorado College.

Includes bibliographical references.

Colorado College Honor Code upheld.

Administrative Notes

The author has given permission for this work to be deposited in the Digital Archive of Colorado College.

Colorado College Honor Code upheld.

Copyright
Copyright restrictions apply.
Publisher
Colorado College
PID
coccc:9549
Digital Origin
born digital
Extent
27 pages : illustrations (some color)
Thesis
Senior Thesis -- Colorado College
Thesis Advisor
Lostroh, Phoebe Daugherty, Margaret
Department/Program
Biology
Degree Name
bachelor
Degree Type
B.A.
Degree Grantor
Colorado College
Date Issued
2014-05