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The GASP phenotype in Acinetobacter baylyi

by Krych, Leland William

Abstract

Competence for natural transformation is the physiological ability of bacteria to take up extracellular DNA. This ability is wielded by over 40 species of bacteria. We hypothesize that competence for natural transformation might be required for the GASP phenotype: a phenomenon by which cells grown to long term stationary phase can out-compete young cells co-incubated with them in stationary phase. In this experiment, we show for the first time that the soil bacteria Acinetobacter baylyi exhibits the GASP phenotype, and that knock out mutants of the gene comP which codes for a prepilin-like protein showed significantly reduced fitness levels during a GASP test when competed against young wild type cells. Knock out mutants of the gene comQ, which codes for a transmembrane protein, showed reduced fitness levels when competed against a young wild type strain in stationary phase but this difference was not significant. Defective GASP responses suggest that natural competence is important for cells to “age” (evolve) normally during long-term stationary phase.

Note

Colorado College Honor Code upheld.

Includes bibliographical references.

Alternative title supplied by cataloger.

Administrative Notes

Colorado College Honor Code upheld.

Alternative title supplied by cataloger.

Copyright
Copyright restrictions apply.
Publisher
Colorado College
PID
coccc:8029
Digital Origin
born digital
Extent
24 pages
Thesis
Senior Thesis -- Colorado College
Thesis Advisor
Lostroh, Phoebe
Department/Program
Biology
Degree Name
bachelor
Degree Type
Bachelor of Arts
Degree Grantor
Colorado College
Date Issued
2013